Catégories : Tous - enzymes - pressure - chemicals

par Aneesh Risbud Il y a 5 années

259

CELL DISRUPTION

The text delves into various physico-mechanical and chemical methods for cell disruption, which is crucial for processes like enzyme purification. Physico-mechanical techniques include agitation with abrasives, using rotating discs and beads made of materials such as glass, alumina ceramics, and titanium, often cooled by a jacket to manage heat dissipation.

CELL DISRUPTION

CELL DISRUPTION

CHEMICAL METHODS

ENZYME TREATMENT
USED FOR [RETREATMENT
EXPENSIVE AND PRESENCE COMPLICATE DOWNSTREAM
HYDROLYZE SPECIFIC BONDS BY ENZYMES

TRICODERMA

PENICILLIUM

MICROMONOSPORA

LEUCOCYTES

STREPTOMYCES

OSMOTIC SHOCK
EFFECT IS MINIMAL
CAUSES SUDDEN CHANGE IN SALT CONC.
DETERGENTS AND ALKALI TREATMENTS
ALKALI

ENZYME

20-30 MINS

pH- 11.5-12.5

HYDROLYSIS OF CELL WALL

DETERGENTS

PROTEIN DENATURATION

COMPOUNDS

TRITON X-100

SODIUM LAURYL SULPHATE

SDS

QUART AMMONIUM

DAMAGE LIPOPROTEIN

PHYSICO-MECHANICAL METHODS

ULTRASONICATION
VOLUMES UPTO 10 CM3 ARE EFFECTIVE
PROBES

SHORT RANGE

SHORT WORK LIFE

20 kHz VIBRATION LEADING TO CAVITATION AND SHOCK WAVES
FREEZE THAWING
SLOW , LIMITED RELEASE OF CELLULAR MATERIALS
THAWING CAUSES DISRUPTION
OF MICROBIAL CELL PASTE- ICE CRYSTAL FORMATION
AGITATION WITH ABRASIVES
DISSIPATION OVERCOME BY COOLING JACKET
BEADS- GLASS, ALUMINA CERAMICS AND TITANIUM
ROTATING DISCS AND SMALL BEADS IN A DISINTEGRATOR
SOLID SHEAR
DISRUPTION DUE TO ICE CRYSTALS
IDEAL FOR TEMPRATURE LABILE
PRESSURE EXTRUSION THROUGH ORIFICE AT -25 C
LIQUID SHEAR
WORKS ON PRINCIPLE OF PRESSURE DROP ACROSS VALVES
IMPINGES AGAINST OPERATIVE PRESSURE
HIGH PRESSURE HOMOGENIZERS EFFECTIVE FOR MICROBIAL DISRUPTION
LARGE SCALE ENZYME PURIFICATIOM
SLURRY PASSES THROUGH NO RETURN VALVES